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Article Navigation> JOURNAL OF BEIJING INSTITUTE OF TECHNOLOGY> 2016> 25(2): 295-300
ZHANG Mei, SHAN Ke-ming, XU Wei, LIN Fan-kai, CHEN Zhao, DENG Yu-lin. Establishment of in vitro human aging model and primary screening of aging peptide-biomarkers[J]. JOURNAL OF BEIJING INSTITUTE OF TECHNOLOGY, 2016, 25(2): 295-300. doi: 10.15918/j.jbit1004-0579.201625.0219
Citation: ZHANG Mei, SHAN Ke-ming, XU Wei, LIN Fan-kai, CHEN Zhao, DENG Yu-lin. Establishment of in vitro human aging model and primary screening of aging peptide-biomarkers[J].JOURNAL OF BEIJING INSTITUTE OF TECHNOLOGY, 2016, 25(2): 295-300.doi:10.15918/j.jbit1004-0579.201625.0219
shu

Establishment of in vitro human aging model and primary screening of aging peptide-biomarkers

doi:10.15918/j.jbit1004-0579.201625.0219
  • 1.

    State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China;National Institute of Communicable Diseases Control and Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China;School of Life Science, Beijing Institute of Technology, Beijing 100081, China;Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China

  • 2.

    Planning and Research Institute, Norinco Group, Beijing 100053, China

  • 3.

    School of Life Science, Beijing Institute of Technology, Beijing 100081, China

  • 4.

    Department of Sociology, School of Social Science, Tsinghua University, Beijing 100084, China

  • Received Date:2014-11-11
    Abstract
  • Aging refers to a multidimensional process that all changes were accumulated in a person over time. These aging changes are associated with progressive increases in the chance of disease and death. Thus, it is necessary to establish a model to screen biomarkers to characterize and evaluate aging degree. In this study, an in vitroaging model was set up by formaldehdye and human serum albumin (HSA), the most abundant protein in human plasma, based on Maillard Reaction. The liquid chromatography tandem mass spectrometry (LC-MS/MS) method with 18O-labeling technique was employed to quantify modification degree of peptides cleaved from HSA. This model was established and optimized for further quantitative biomarker study.
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