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Volume 31Issue 3
Jun. 2022
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Article Navigation> JOURNAL OF BEIJING INSTITUTE OF TECHNOLOGY> 2022> 31(3): 275-284
Dianlong Yang, Xiaodan Jiang, Yijie Zhou, Xiaobin Dong, Luyao Liu, Lulu Zhang, Xianbo Qiu. A Microfluidic System with Active Mixing for Improved Real-Time Isothermal Amplification[J]. JOURNAL OF BEIJING INSTITUTE OF TECHNOLOGY, 2022, 31(3): 275-284. doi: 10.15918/j.jbit1004-0579.2022.026
Citation: Dianlong Yang, Xiaodan Jiang, Yijie Zhou, Xiaobin Dong, Luyao Liu, Lulu Zhang, Xianbo Qiu. A Microfluidic System with Active Mixing for Improved Real-Time Isothermal Amplification[J].JOURNAL OF BEIJING INSTITUTE OF TECHNOLOGY, 2022, 31(3): 275-284.doi:10.15918/j.jbit1004-0579.2022.026
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A Microfluidic System with Active Mixing for Improved Real-Time Isothermal Amplification

doi:10.15918/j.jbit1004-0579.2022.026

  • College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China

Funds:This work was supported by the National Natural Science Foundation of China (Nos. 81871505, 61971026), the Fundamental Research Fund for the Central Universities (No. XK1802-4), the National Science and Technology Major Project (No. 2018ZX10732101-001-009), and the Research Fund to the Top Scientific and Technological Innovation Team from Beijing University of Chemical Technology (No. buctylkjcx06).
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  • Author Bio:

    Dianlong Yangis a graduate student in College of Information Science and Technology, Beijing University of Chemical Technology. He obtained his bachelor’s degree from Beijing University of Chemical Technology. His current research interests include biomedical instrumentation in point-of-care diagnosis and microfluidics

    Xiaodan Jiangis a graduate student in College of Information Science and Technology, Beijing University of Chemical Technology, China. She obtained her bachelor’s degree from Beijing University of Chemical Technology. Her current research interests include microfluidics and convection PCR

    Yijie Zhouis an undergraduate student in College of Information Science and Technology, Beijing University of Chemical Technology, China. His current research interests include biomedical instrumentation in point-of-care diagnosis and microfluidics

    Xiaobin Dongobtained his master’s degree from Shenzhen University in 2018. Currently, he is a Ph.D. student in College of Information Science and Technology, Beijing University of Chemical Technology. His research focuses on the microfluidics PCR and lab-on-a-disc microfluidics

    Luyao Liureceived M.S. degree in Mechanical and Aerospace Engineering from George Washington University in 2014. He is currently a Ph.D. candidate in College of Information Science and Technology, Beijing University of Chemical Technology. His main research interests are microfluidic and biomedical device

    Lulu Zhangreceived her Ph.D. in microelectronics and solid state electronics from University of Chinese Academy of Sciences in 2014, China. She was a visiting scholar in University of Pennsylvania from 2017 to 2018. She is an associate professor in Department of Measurement and Control in Beijing University of Chemical Technology, China. Her current research interests include biochemical sensors and microsystems, especially surface plasma resonance sensors and instruments

    Xianbo Qiureceived his Ph.D. in control theory and control engineering from Shanghai Jiaotong University in 2006, China. He was a postdoctor researcher in University of Pennsylvania from 2006 to 2011. He is a professor in Department of Automation in Beijing University of Chemical Technology, China. His current research interests include microfluidics, lab-on-chip system, and biomedical instrumentation in point-of-care diagnosis

  • Corresponding author:xbqiu@mail.buct.edu.cn
  • Received Date:2022-03-08
  • Rev Recd Date:2022-04-17
  • Accepted Date:2022-05-05
  • Publish Date:2022-06-28
    Abstract
  • To improve the performance of real-time recombinase polymerase amplification (RPA), a microfluidic system with active mixing is developed to optimize the reaction dynamics. Instead of adopting a single typical reaction chamber, a specific reactor including a relatively large chamber in center with two adjacent zig-zag channels at two sides is integrated into the microfluidic chip. Active mixing is achieved by driving the viscous reagent between the chamber and the channel back and forth periodically with an outside compact peristaltic pump. To avoid reagent evaporation, one end of the reactor is sealed with paraffin oil. A hand-held companion device is developed to facilitate real-time RPA amplification within 20 min. The whole area of the reactor is heated with a resistance heater to provide uniform reaction temperature. To achieve real-time monitoring, a compact fluorescence detection module is integrated into the hand-held device. A smartphone with custom application software is adopted to control the hand-held device and display the real-time fluorescence curves. The performances of two cases with and without active on-chip mixing are compared between each other by detecting African swine fever viruses. It has been demonstrated that, with active on-chip mixing, the amplification efficiency and detection sensitivity can be significantly improved.
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