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SHI Ying, YAO Guo-wei, OU Yu-xiang, MA Min. Determination of 9-Deoxo-9a-Aza-9a-Homoerythromycin A and Related Substances by High-Performance Liquid Chromatography[J]. JOURNAL OF BEIJING INSTITUTE OF TECHNOLOGY, 2005, 14(4): 420-424.

Citation:

SHI Ying, YAO Guo-wei, OU Yu-xiang, MA Min. Determination of 9-Deoxo-9a-Aza-9a-Homoerythromycin A and Related Substances by High-Performance Liquid Chromatography[J].JOURNAL OF BEIJING INSTITUTE OF TECHNOLOGY, 2005, 14(4): 420-424.

Citation:

SHI Ying, YAO Guo-wei, OU Yu-xiang, MA Min. Determination of 9-Deoxo-9a-Aza-9a-Homoerythromycin A and Related Substances by High-Performance Liquid Chromatography[J].JOURNAL OF BEIJING INSTITUTE OF TECHNOLOGY, 2005, 14(4): 420-424.

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Determination of 9-Deoxo-9a-Aza-9a-Homoerythromycin A and Related Substances by High-Performance Liquid Chromatography

1.
School of Material Science and Engineering, Beijing Institute of Technology, Beijing 100081, China; School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China
2.
School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China
3.
School of Material Science and Engineering, Beijing Institute of Technology, Beijing 100081, China

Received Date:2004-05-14

Abstract

Abstract

A high-performance liquid chromatography(HPLC) method was developed and validated for the assay of 9-deoxo-9a-aza-9a-homoerythromycin A and related substances that might coexist in products as impurities that originate from the synthesis processes. A chromatographic system comprising an ODS 150 mm× 4.6 mm I.D. column, a mobile phase of cetonitrile monobasic potassium phosphate buffer (25/75), a flow rate of 1.2 mL/min, a temperature of 30 ℃ and a UV detector set at 205 nm has shown good chromatographic separation for 9-deoxo-9a-aza-9a-homoerythromycin A and other related substances. The linearity of the calibration curves, the precision, expressed as relative standard deviations, of the HPLC method have been studied. The HPLC method under study was found to be specific, precise, accurate and reproducible, indicating stability.
- 9-deoxo-9a-aza-9a-homoerythromycin A,
- related substances,
- HPLC

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References (12)

References

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